High Performance Liquid Chromatography:Article Title: Compositions and methods of PGL for the increased production of isoprene
Article Snippet: .. The mixture was then centrifuged for 5 minutes at 14,000×g and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm)(Catalog #125-0140)(BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm)(Catalog #125-0129)(BioRad); (4) running buffer=0.01N H2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=100 microliters; (8) runtime=26 minutes. ..
Article Title: Recombinant microorganisms for enhanced production of mevalonate, isoprene, and isoprenoids
Article Snippet: .. Next, the sample was centrifuged at 14,000×g for 5 minutes and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm) (Catalog #125-0140) (BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm) (Catalog #125-0129) (BioRad); (4) running buffer=0.01N H2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=100 microliters; (8) runtime=26 minutes. (iii) Results Strain CMP678 (GI1.2gltA) was assessed using strain CMP694 (parent) as a control. .. Next, the sample was centrifuged at 14,000×g for 5 minutes and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm) (Catalog #125-0140) (BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm) (Catalog #125-0129) (BioRad); (4) running buffer=0.01N H2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=100 microliters; (8) runtime=26 minutes. (iii) Results Strain CMP678 (GI1.2gltA) was assessed using strain CMP694 (parent) as a control.
Article Title: Utilization of phosphoketolase in the production of mevalonate, isoprenoid precursors, and isoprene
Article Snippet: .. Next, the sample was centrifuged at 14,000×g for 5 minutes and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm)(Catalog #125-0140)(BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm) (Catalog #125-0129) (BioRad); (4) running buffer=0.01N H2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=20 microliters; (8) runtime=26 minutes. (iii) Results: Strains expressing phosphoketolase grew slower than the control strain (FIG. 9). ..
Article Title: Recombinant microorganisms for enhanced production of mevalonate, isoprene, and isoprenoids
Article Snippet: .. Next, the sample was centrifuged at 14,000×g for 5 minutes and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm) (Catalog #125-0140) (BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm) (Catalog #125-0129) (BioRad); (4) running buffer=0.01N H2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=100 microliters; (8) runtime=26 minutes. (iii) Results Strains MD10-555 (PL.6pdh), CMP711 (GI1.2maeB) and CMP729 (pdhR) were assessed using strain CMP678 (parent) as a control. .. Next, the sample was centrifuged at 14,000×g for 5 minutes and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm) (Catalog #125-0140) (BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm) (Catalog #125-0129) (BioRad); (4) running buffer=0.01N H2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=100 microliters; (8) runtime=26 minutes. (iii) Results Strains MD10-555 (PL.6pdh), CMP711 (GI1.2maeB) and CMP729 (pdhR) were assessed using strain CMP678 (parent) as a control.
Article Title: Use of recombinant PGL for increased production of isoprene
Article Snippet: .. The mixture was then centrifuged for 5 minutes at 14,000×g and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm)(Catalog #125-0140) (BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm)(Catalog #125-0129)(BioRad); (4) running buffer=0.01NH2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=100 microliters; (8) runtime=26 minutes. ..
Injection:Article Title: Compositions and methods of PGL for the increased production of isoprene
Article Snippet: .. The mixture was then centrifuged for 5 minutes at 14,000×g and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm)(Catalog #125-0140)(BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm)(Catalog #125-0129)(BioRad); (4) running buffer=0.01N H2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=100 microliters; (8) runtime=26 minutes. ..
Article Title: Recombinant microorganisms for enhanced production of mevalonate, isoprene, and isoprenoids
Article Snippet: .. Next, the sample was centrifuged at 14,000×g for 5 minutes and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm) (Catalog #125-0140) (BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm) (Catalog #125-0129) (BioRad); (4) running buffer=0.01N H2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=100 microliters; (8) runtime=26 minutes. (iii) Results Strain CMP678 (GI1.2gltA) was assessed using strain CMP694 (parent) as a control. .. Next, the sample was centrifuged at 14,000×g for 5 minutes and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm) (Catalog #125-0140) (BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm) (Catalog #125-0129) (BioRad); (4) running buffer=0.01N H2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=100 microliters; (8) runtime=26 minutes. (iii) Results Strain CMP678 (GI1.2gltA) was assessed using strain CMP694 (parent) as a control.
Article Title: Utilization of phosphoketolase in the production of mevalonate, isoprenoid precursors, and isoprene
Article Snippet: .. Next, the sample was centrifuged at 14,000×g for 5 minutes and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm)(Catalog #125-0140)(BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm) (Catalog #125-0129) (BioRad); (4) running buffer=0.01N H2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=20 microliters; (8) runtime=26 minutes. (iii) Results: Strains expressing phosphoketolase grew slower than the control strain (FIG. 9). ..
Article Title: Recombinant microorganisms for enhanced production of mevalonate, isoprene, and isoprenoids
Article Snippet: .. Next, the sample was centrifuged at 14,000×g for 5 minutes and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm) (Catalog #125-0140) (BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm) (Catalog #125-0129) (BioRad); (4) running buffer=0.01N H2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=100 microliters; (8) runtime=26 minutes. (iii) Results Strains MD10-555 (PL.6pdh), CMP711 (GI1.2maeB) and CMP729 (pdhR) were assessed using strain CMP678 (parent) as a control. .. Next, the sample was centrifuged at 14,000×g for 5 minutes and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm) (Catalog #125-0140) (BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm) (Catalog #125-0129) (BioRad); (4) running buffer=0.01N H2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=100 microliters; (8) runtime=26 minutes. (iii) Results Strains MD10-555 (PL.6pdh), CMP711 (GI1.2maeB) and CMP729 (pdhR) were assessed using strain CMP678 (parent) as a control.
Article Title: Use of recombinant PGL for increased production of isoprene
Article Snippet: .. The mixture was then centrifuged for 5 minutes at 14,000×g and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm)(Catalog #125-0140) (BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm)(Catalog #125-0129)(BioRad); (4) running buffer=0.01NH2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=100 microliters; (8) runtime=26 minutes. ..
Control:Article Title: Recombinant microorganisms for enhanced production of mevalonate, isoprene, and isoprenoids
Article Snippet: .. Next, the sample was centrifuged at 14,000×g for 5 minutes and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm) (Catalog #125-0140) (BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm) (Catalog #125-0129) (BioRad); (4) running buffer=0.01N H2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=100 microliters; (8) runtime=26 minutes. (iii) Results Strain CMP678 (GI1.2gltA) was assessed using strain CMP694 (parent) as a control. .. Next, the sample was centrifuged at 14,000×g for 5 minutes and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm) (Catalog #125-0140) (BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm) (Catalog #125-0129) (BioRad); (4) running buffer=0.01N H2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=100 microliters; (8) runtime=26 minutes. (iii) Results Strain CMP678 (GI1.2gltA) was assessed using strain CMP694 (parent) as a control.
Article Title: Utilization of phosphoketolase in the production of mevalonate, isoprenoid precursors, and isoprene
Article Snippet: .. Next, the sample was centrifuged at 14,000×g for 5 minutes and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm)(Catalog #125-0140)(BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm) (Catalog #125-0129) (BioRad); (4) running buffer=0.01N H2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=20 microliters; (8) runtime=26 minutes. (iii) Results: Strains expressing phosphoketolase grew slower than the control strain (FIG. 9). ..
Article Title: Recombinant microorganisms for enhanced production of mevalonate, isoprene, and isoprenoids
Article Snippet: .. Next, the sample was centrifuged at 14,000×g for 5 minutes and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm) (Catalog #125-0140) (BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm) (Catalog #125-0129) (BioRad); (4) running buffer=0.01N H2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=100 microliters; (8) runtime=26 minutes. (iii) Results Strains MD10-555 (PL.6pdh), CMP711 (GI1.2maeB) and CMP729 (pdhR) were assessed using strain CMP678 (parent) as a control. .. Next, the sample was centrifuged at 14,000×g for 5 minutes and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm) (Catalog #125-0140) (BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm) (Catalog #125-0129) (BioRad); (4) running buffer=0.01N H2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=100 microliters; (8) runtime=26 minutes. (iii) Results Strains MD10-555 (PL.6pdh), CMP711 (GI1.2maeB) and CMP729 (pdhR) were assessed using strain CMP678 (parent) as a control.
Expressing:Article Title: Utilization of phosphoketolase in the production of mevalonate, isoprenoid precursors, and isoprene
Article Snippet: .. Next, the sample was centrifuged at 14,000×g for 5 minutes and the supernatant collected for HPLC analysis run under the following conditions: (1) BioRad—Aminex HPX-87H Ion Exclusion Column (300 mm×7.8 mm)(Catalog #125-0140)(BioRad, Hercules, Calif.); (2) column temperature=50° C.; (3) BioRad—Microguard Cation H guard column refill (30 mm×4.6 mm) (Catalog #125-0129) (BioRad); (4) running buffer=0.01N H2SO4; (5) running buffer flow rate=0.6 ml/min; (6) approximate running pressure= ̃950 psi; (7) injection volume=20 microliters; (8) runtime=26 minutes. (iii) Results: Strains expressing phosphoketolase grew slower than the control strain (FIG. 9). ..
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